Animal Care, Surgery, and Pathology Core

The Core provides extensive scientific support services, including importation/exportation, colony management, customized mouse models, rederivation and creation of genetically-modified mutant mice (e.g. CRISPR), and re-animation of a variety of models cryopreserved within the KOMP or MMRRC repositories. These new and extant mouse models can then be bred to produce male and female cohorts for in vivo and in vitro phenotyping, including rederivation into our Gnotobiotic Mouse Research Center for gut microbiome studies.

The Core also operates a Microsurgery Suite, with two expert microsurgeons, for development of surgically-manipulated mouse models, such as bariatric surgical models, e.g. Vertical Sleeve Gastrectomy (VSG) and Roux en Y Gastric Bypass (RYGB), as well as cannulation telemetry and osmotic pump implants, in addition to providing microsurgery training services for investigators and clinicians. In addition to testing, manipulation, and sampling, investigator can have these surgically-manipulated models as well as any genetically-engineered mouse lines shipped from our approved vendor barrier vivarium directly into the recipient institution’s vivarium.

The Core also provides ancillary services and procedures, including blood sampling for in vivo testing for glucose tolerance tests and insulin tolerance tests.   We provide pathology analysis to complement other testing including survey necropsy with histology, clinical chemistry and hematology analysis. This testing can be customized to include specific organ histologic examination, grading and photo documentation.

The Core is led by Kristin Grimsrud DVM, PhD, who oversees two trained rodent surgical technicians, one import/export coordinator, and two phenotyping technicians, and is co-led by Stephen Griffey DVM, PhD.